牦牛Nramp1基因mRNA组织表达谱及其相关miRNAs初步研究

doi:10.11843/j.issn.0366-6964.2019.08.006

Abstract

Abstract: The aim of this study was to investigate the tissue expression profiles of yak Nramp1 gene mRNA and the possible miRNAs targeting to Nramp1. The mRNA expression profile of yak Nramp1 gene was analyzed by RT-PCR, and the possible miRNAs targeting to Nramp1 were predicted by TargentScan and miRBase softwares, and the relative expression levels for these miRNAs were analyzed in the 10 yak tissues including liver, spleen, lung, kidney, hind leg skeletal muscle, ovary, small intestine, submandibular lymph node, large intestine and intestinal lymph nodes by adding PloyA tail RT-PCR method. The results showed that the Nramp1 gene mRNA was expressed in the 10 examined tissues, and the expression levels in the yak spleen, submandibular lymph node and lung were extremely significantly higher than that in the liver, kidney, large intestine and intestinal lymph node(P<0.01), and significantly higher than that in small intestine tissue (P<0.05). A total of 201 miRNAs that might target to the yak Nramp1 gene were predicted, and 6 of them were selected for expression profiling analysis. It was found that Nramp1 mRNA and bta-miR-106a, bta-miR-20b, bta-miR-17-5p were co-expressed in the yak immune tissues such as the spleen, submandibular lymph node and intestinal lymph node, and were co-expressed with bta-miR-93, bta-miR-106b and bta-miR-20a in yak submandibular lymph nodes and intestinal lymph nodes. There were no significant differences in the expression of bta-miR-93,bta-miR-20a,bta-miR-106a,bta-miR-17-5p and bta-miR-20b between submandibular lymph nodes and intestinal lymph nodes(P>0.05), but the expression of bta-miR-106a and bta-miR-20b in submandibular lymph nodes and intestinal lymph nodes were extremely significantly higher than those in spleen tissues(P<0.01),and the expression of bta-miR-106b in submandibular lymph nodes was extremely significantly higher than that in intestinal lymph nodes(P<0.01). The extensive expression of Nramp1 mRNA in various tissues of yak suggested that it might have a wide range of immunomodulatory effects, and the co-expression of bta-miR-93, bta-miR-20b, bta-miR-106a, bta-miR-106b, bta-miR-20a, bta-miR-17-5p and Nramp1 mRNA in immune tissues indicated that they might have targeted regulatory relationship in immune regulation, but whether there is targeting regulation and their regulation mechanism remains to be further studied.

CLC Number:

S823.2

WEN Dongxu, ZHANG Lei, SUOLANG Sizhu, NIU Jiaqiang, WANG Yuheng, GONG Ga, DANBA Ciren, XI Guangyin, GUO Min, XU Yefen. Preliminary Study on Tissue Expression Profile and Related miRNAs of Yak Nramp1 Gene[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(8): 1576-1586.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2019.08.006

https://www.xmsyxb.com/EN/Y2019/V50/I8/1576

References

[1] PERACINO B,BURACCO S,BOZZARO S.The Nramp (Slc11) proteins regulate development,resistance to pathogenic bacteria and iron homeostasis in Dictyostelium discoideum[J].J Cell Sci,2013,126:301-311.
[2] DAR M A,AHMED R,URWAT U,et al.Expression kinetics of natural resistance associated macrophage protein (NRAMP) genes in Salmonella Typhimurium-infected chicken[J].BMC Vet Res,2018,14(1):180.
[3] NEVES J V,WILSON J M,KUHL H,et al.Natural history of SLC11 genes in vertebrates:tales from the fish world[J].BMC Evol Biol,2011,11:106.
[4] SOPHIE M,HAMEED A,MUNEER A,et al.SLC11A1 polymorphisms and host susceptibility to cutaneous leishmaniasis in Pakistan[J]. Parasit Vect,2017,10:12.
[5] CHEN S L,XU M Y,JI X S,et al.Cloning and characterisation of natural resistance associated macrophage protein (Nramp) cDNA from red sea bream (Pagrus major)[J].Fish Shellfish Immunol,2004,17(4):305-313.
[6] POWELL D A,FRELINGER J A.Efficacy of resistance to Francisella imparted by ITY/NRAMP/SLC11A1 depends on route of infection[J]. Front Immunol,2017,8:206.
[7] JIANG Y H,MAO Y,LV Y N,et al.Natural resistance associated macrophage protein is involved in immune response of blunt snout bream,Megalobrama amblycephala[J].Cells,2018,7(4):27.
[8] WORKU M,MORRIS A.Binding of different forms of lipopolysaccharide and gene expression in bovine blood neutrophils[J].J Dairy Sci, 2009,92(7):3185-3193.
[9] ALI O S,SHABAYEK M I,SELEEM M M,et al.microRNAs 182 and 375 sera expression as prognostic biochemical markers in breast cancer[J].Clin Breast Cancer,2018,18(6):e1373-e1379.
[10] BRONEVETSKY Y,ANSEL K M.Regulation of miRNA biogenesis and turnover in the immune system[J].Immunol Rev,2013, 253(1):304-316.
[11] CHEN X,YIN J,QU J,et al.MDHGI:matrix decomposition and heterogeneous graph inference for miRNA-disease association prediction[J].PLoS Comput Biol,2018,14(8):e1006418.
[12] DONG H,JIANG W,CHEN H Q,et al.microRNA-145 attenuates IL-6-induced enhancements of sensitivity to UVB irradiation by suppressing MyD88 in HaCaT cells[J].Int J Immunopathol Pharmacol,2018,32:2058738418795940.
[13] BALUNI M,GHILDIYAL S,SINGHD,et al.Increased serum microRNA-29b expression and bad recovery in Japanese encephalitis virus infected patients;a new component to improve the disease recovery[J].J Neuroimmunol,2018,323:56-61.
[14] WANG K,SHEN X L,JIA J S,et al.High-throughput sequencing analysis of microRNAs in gills of red swamp crayfish, Procambarus clarkii infected with white spot syndrome virus[J].Fish Shellfish Immunol,2018,83:18-25.
[15] LI M,HUANG Q,WANG J F,et al.Differential expression of microRNAs in Portunus trituberculatus in response to Hematodinium parasites[J]. Fish Shellfish Immunol,2018,83:134-139.
[16] DU Q,WU X C,WANG T T,et al.Porcine circovirus type 2 suppresses IL-12p40 induction via capsid/gC1qR-mediated microRNAs and signalings[J].J Immunol,2018,201(2):533-547.
[17] BROGAARD L,LARSEN L E,HEEGAARD P M H,et al.IFN-λ and microRNAs are important modulators of the pulmonary innate immune response against influenza A (H1N2) infection in pigs[J].PLoS One,2018,13(4):e0194765.
[18] 赵冬雪,穆卫涛,王立砺,等.禽流感病毒与microRNA[J].中国兽医学报,2017,37(8):1613-1616.
ZHAO D X,MU W T,WANG L L,et al.Avian influenza virus and microRNA[J].Chinese Journal of Veterinary Science,2017, 37(8):1613-1616.(in Chinese)
[19] 赵冬雪,穆卫涛,王立砺,等.传染性法氏囊病病毒与microRNA(miRNA)[J].中国兽医学报,2017,37(4):762-767.
ZHAO D X,MU W T,WANG L L,et al.Infectious bursal disease virus and microRNA (miRNA)[J].Chinese Journal of Veterinary Science,2017,37(4):762-767.(in Chinese)
[20] 江船.牛流行热病毒感染宿主细胞差异表达miRNA的筛选及其鉴定[D].济南:山东大学,2018.
JIANG C.The screening and identification of differentially expressed miRNAs of bovine ephemeral fever virus infected host cells[D].Jinan:Shandong University,2018.(in Chinese)
[21] 付强.牛病毒性腹泻病毒持续性感染的分子机制研究及其定点突变株的构建[D].石河子:石河子大学,2015.
FU Q.Study on the molecular mechanisms of persistent replication of bovine viral diarrhea virus and construction of site-directed mutation of bovine viral diarrhea virus[D].Shihezi:Shihezi University,2015.(in Chinese)
[22] 王军,燕晓晓,丁赫,等.LPS诱导牛子宫内膜细胞miRNA差异表达分析[J].中国兽医杂志,2017,53(10):10-12,16.
WANG J,YAN X X,DING H,et al.Lipopolysaccharide-induced differential expression of miRNAs in bovine endometrial cells[J].Chinese Journal of Veterinary Medicine,2017,53(10):10-12,16.(in Chinese)
[23] 王玉恒,徐业芬,牛家强,等.牦牛Smad 4基因mRNA组织表达谱及其miRNAs初步研究[J].畜牧兽医学报,2017,48(10):1882-1891.
WANG Y H,XU Y F,NIU J Q,et al.Preliminary studies on tissue expression profiles of Smad 4 mRNA and its miRNAs in yak[J].Acta Veterinaria et Zootechnica Sinica,2017,48(10):1882-1891.(in Chinese)
[24] 廖珂.牦牛、普通牛和犏牛睾丸组织microRNA的比较研究[D].成都:西南民族大学,2016.
LIAO K.Comparative analysis of microRNA in yak,ordinary cattle and cattle-yak testis tissue[D].Chengdu:Southwest Minzu University,2016.(in Chinese)
[25] 熊显荣,兰道亮,李键,等.牦牛卵巢小RNA高通量测序及生物信息学分析[J].畜牧兽医学报,2016,47(1):55-63.
XIONG X R,LAN D L,LI J,et al.Solexa sequencing of small RNAs in yak (Bos grunniens) ovaries and bioinformatics analysis[J]. Acta Veterinaria et Zootechnica Sinica,2016,47(1):55-63.(in Chinese)
[26] 李明霞,潘和平,阎萍,等.牦牛GDF9和BMP15基因的克隆、表达分析及miRNA研究[J].生物技术通报,2016,32(2):100-108.
LI M X,PAN H P,YAN P,et al.Cloning,expression analysis and miRNA study of gene GDF9 and BMP15 in yak[J].Biotechnology Bulletin,2016,32(2):100-108.(in Chinese)
[27] CELLIER M F M.Nutritional immunity:homology modeling of Nramp metal import[M]//LAMBRIS J D,HAJISHENGALLIS G.Current Topics in Innate Immunity Ⅱ.New York,NY:Springer,2012:335-351.
[28] HU Y,SHAN Y J,ZHU C H,et al.Upregulation of NRAMP1 mRNA confirms its role in enhanced host immunity in post-artificial infections of Salmonella enteritidis in chicks[J].Br Poult Sci,2015,56(4):408-415.
[29] FRITSCHE G,NAIRZ M,LIBBY S J,et al.Slc11a1(Nramp1) impairs growth of Salmonella enterica serovar typhimurium in macrophages via stimulation of lipocalin-2 expression[J].J Leukoc Biol,2012,92(2):353-359.
[30] DAI C H,WU J Y,ZHAO C X,et al.Nramp1 gene expression in different tissues of Meishan piglets from newborn to weaning[J]. Genet Mol Res, 2017,16(1):gmr16019288.
[31] 应三成.藏猪几个重要遗传特性基因的研究[D].成都:四川大学,2007.
YING S C.Study on genes related to several important genetic traits in Tibetan pig[D].Chengdu:Sichuan University,2007.(in Chinese)
[32] 胡国顺.鸡Nramp1基因的遗传变异、表达以及与部分免疫性状的关联分析[D].扬州:扬州大学,2011.
HU G S.Genetic variation and expression of Nramp1 gene and its association with chicken immune qualities[D].Yangzhou:Yangzhou University,2011.(in Chinese)
[33] OH S,PARK M R,SON S J,et al.Comparison of total RNA isolation methods for analysis of immune-related microRNAs in Market Milks[J].Korean J Food Sci Anim Resour,2015,35(4):459-465.
[34] SHYAMASUNDAR S,LIM J P,BAY B H.miR-93 inhibits the invasive potential of triple-negative breast cancer cells in vitro via protein kinase WNK1[J].Int J Oncol,2016,49(6):2629-2636.
[35] WU M Z,CHENG W C,CHEN S F,et al.miR-25/93 mediates hypoxia-induced immunosuppression by repressing cGAS[J].Nat Cell Biol, 2017,19(10):1286-1296.
[36] NI Q T,STEVIC I,PAN C,et al.Different signatures of miR-16,miR-30b and miR-93 in exosomes from breast cancer and DCIS patients[J].Sci Rep,2018,8(1):12974.
[37] KÄSTLE M,BARTEL S,GEILLINGER-KÄSTLE K,et al.microRNA cluster 106a-363 is involved in T helper 17 cell differentiation[J]. Immunology,2017,152(3):402-413.
[38] OH S,PARK M R,RYU S,et al.Quantitative analysis of milk-derived micrornas and microbiota during the manufacturing and ripening of soft cheese[J].J Microbiol Biotechnol,2017,27(9):1566-1575.
[39] LIANG X,ZHAO Q H,GENG T T,et al.miR-106b regulates the apoptosis and tumorigenesis of hepatocellular carcinoma via targeting Zinc finger and BTB domain-containing protein 7A (Zbtb7a)[J].J Biochem Mol Toxicol,2018,32(8):e22169.
[40] WU H,WANG F L,HU S L,et al.miR-20a and miR-106b negatively regulate autophagy induced by leucine deprivation via suppression of ULK1 expression in C2C12 myoblasts[J].Cell Signal,2012,24(11):2179-2186.
[41] HONG S B,YU S,LI J,et al.miR-20b displays tumor-suppressor functions in papillary thyroid carcinoma by regulating the MAPK/ERK signaling pathway[J].Thyroid,2016,26(12):1733-1743.
[42] CHEN Q X,ZHOU R,ZHANG Y C,et al.Bone marrow mesenchymal stromal cells attenuate liver allograft rejection may via upregulation PD-L1 expression through downregulation of miR-17-5p[J].Transpl Immunol,2018,51:21-29.
[43] CHEN H Q,ZHANG D H,ZHANG G P,et al.A semantics-oriented computational approach to investigate microRNA regulation on glucocorticoid resistance in pediatric acute lymphoblastic leukemia[J].BMC Med Inform Decis Mak,2018,18(Suppl 2):57.

Preliminary Study on Tissue Expression Profile and Related miRNAs of Yak Nramp1 Gene

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	MIAO Shu, AN Jishan, WANG Zuo, XIAO Dingfu, LAN Xinyi, LIU Lei, SHEN Weijun, WAN Fachun. Leucine Promotes the Proliferation of Bovine Myoblasts through PI3K-AKT Signaling Pathway [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 142-152.
[2]	NIU Yifan, YANG Baigao, ZHANG Peipei, ZHANG Hang, FENG Xiaoyi, CAO Jianhua, YU Zhou, HAO Haisheng, DU Weihua, ZOU Huiying, ZHU Huabin, MA Youji, ZHAO Xueming. Advances in Bovine Embryo Genome Selection [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4449-4457.
[3]	WANG Wenxiang, DU Lili, HU Junwei, ZHANG Yanxiang, MA Minghao, DUAN Rui, QIAN Cong, WANG Xinyue, LI Sanlu, ZHANG Changqing, ZHANG Lupei, GAO Xue, XU Lingyang, LI Junya, GAO Huijiang. Mining Candidate Genes Related to Meat Quality Traits of Longissimus Lumborum in Pingliang Red Steer Based on Transcriptome [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4589-4604.
[4]	MA Haoran, ZHANG Lupei, JIN Shengyun, BAO Jinshan, LI Hongyan, GAO Huijiang, XU Lingyang, WANG Zezhao, LI Junya. Assessment of the Genomic Relationships for Chinese Indigenous Beef Cattle Using High-density SNP Chip [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4174-4185.
[5]	ZHANG Junxing, ZHANG Hailiang, HAN Liyun, MA Yanfen, WEN Wan, ZHOU Jiamin, TIAN Jia, LU Tingting, MA Yun, WANG Yachun. Analysis on the Influencing Factors of Wellness Traits in Holstein Lactating Cows in Ningxia [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2389-2401.
[6]	YANG Chuang, WU Longfei, LIU Guangbin, LI Yaokun, LIU Dewu, SUN Baoli. Expression Profile and Bioinformatics Analysis of lncRNA and Its Associated ceRNA Networks in Longissimus Dorsi from Lufeng Cattle and Leiqiong Cattle [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 1951-1963.
[7]	GUO Mingpeng, MENG Yuan, WANG Honghao, WANG Yuan, CHE Leijie, SHEN Li, WANG Xi. Estimation of Genetic Parameters of Body Weight and Body Size Traits in Jinnan Cattle at Different Growth Stages [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1452-1464.
[8]	YU Shiqiang, LI Liuxue, ZHAO Xiaobo, ZHAO Huiying, TU Yan, ZHAO Yuchao, JIANG Linshu. Differences and Correlations of Lactation Performance in Chinese Holstein Dairy Cows at Different Lactation Stages and Somatic Levels [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 1003-1014.
[9]	YANG Guang, XU Jing, LI Xin, HU Debao, GUO Yiwen, DING Xiangbin, GUO Hong, ZHANG Linlin. Effect of Interfering lncbMD on Proliferation and Differentiation of Bovine Skeletal Muscle Satellite Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 1015-1025.
[10]	MA Zhijie, WANG Shikang, ZHANG Weizhong, GUO Weixing, ZHAO Haiming, LEI Chuzhao. Y-chromosome Genomic Diversity and Paternal Origins of Qaidam Cattle and Mongolian Cattle [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 1026-1033.
[11]	NIU Ruili, SONG Hanan, WU Yue, WANG Yunan, GAO Yefan, GUAN Weijun, ZONG Xianchun. Isolation, Culture and Biological Characteristics of Epidermal Stem Cells from Simmental Cattle [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(1): 168-177.
[12]	LI Hongwei, XU Lingyang, WANG Zezhao, CAI Wentao, ZHU Bo, CHEN Yan, GAO Xue, ZHANG Lupei, GAO Huijiang, LI Junya. Genome-wide Association Study of Slaughter Traits Based on Haplotype in Beef Cattle [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(12): 4232-4243.
[13]	CHANG Yao, SU Guosheng, LI Yanhua, LI Xiang, MA Zhu, WANG Yachun. Estimating Genetic Parameters for Body Weights using Pedigree and Genotype-pedigree based Approaches in Holstein Heifers [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(11): 3759-3768.
[14]	XU Jingyi, XU Haoqi, HU Lirong, ZHANG Fan, GAO Qing, LUO Hanpeng, ZHANG Hailiang, SHI Rui, LI Xiang, LIU Lin, GUO Gang, WANG Yachun. Association Analysis of MET Gene Single Nucleotide Polymorphism with Reproduction and Milk Production Traits in Chinese Holstein Cattle [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(11): 3769-3785.
[15]	WANG Chuanchuan, MU Tong, FENG Xiaofang, YU Baojun, ZHANG Juan, GU Yaling. Identification of Key Candidate Genes for Milk Fat Metabolism in Dairy Cows Based on Transcriptome Sequencing [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(10): 3421-3433.